Results
| PMID | 15743158 |
| Gene Name | CYP19A1 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 43 |
| Population details | 43 (23 women with endometriosis, 20 fertile cyclic women undergoing tubal sterilization) |
| Sex | Female |
| Associated genes | P450Arom |
| Other associated phenotypes |
Endometriosis |
|
Rev Med Chil. 2004 Dec;132(12):1475-82. Johnson, M Cecilia| Pinto, Claudio| Alves, Alessandra| Palomino, Alberto| Fuentes, Ariel| Boric, M Angelica| Vega, Margarita Instituto de Investigaciones Materno-Infantil, Hospital Clinico San Borja Arriaran, Facultad de Medicina, Universidad de Chile. cjohnson@med.uchile.cl BACKGROUND: Endometriosis, a common gynecologic disorder characterized by endometrial glands and stroma outside the uterus, is diagnosed by direct visualization of peritoneal and ovarian implants during laparoscopy. AIM: To study the estrogenic microenvironment in eutopic endometria of women with and without endometriosis. PATIENTS AND METHODS: Eutopic endometria, obtained during laparoscopy from 23 women with endometriosis and 20 fertile cyclic women undergoing tubal sterilization, was studied. P450Arom mRNA expression (RT-PCR) was measured. Also, P450Arom activity was assessed measuring testosterone conversion to estradiol and the concentration of this last hormone in cultured endometrial explants. RESULTS: Age and body mass index was similar in both groups studied. Seventy nine percent of endometria from women with endometriosis and in 29.4% from control group expressed P450Arom mRNA (p <0.01). The intensity of the band was higher in secretory endometria from women with endometriosis when compared to controls (p <0.01), but it was similar during the proliferative phase. Estradiol secretion to the culture media by proliferative endometria explants from women with endometriosis was 3-fold higher than secretory endometria (p <0.01) and endometria from control women in both phases. P450Arom activity, in the presence of testosterone, was 7-fold higher in endometrial cultures from women with endometriosis, when compare with the basal culture (p <0.01). However, in endometrial explant cultures from control women, this activity was not statistical different. CONCLUSIONS: These results indicate that in women with endometriosis, the microenvironment in the endometria is estrogenic as a consequence of an increased expression and activity of the P450Arom. Mesh Terms: Aromatase/*metabolism| Biopsy| Case-Control Studies| Cells, Cultured| Endometriosis/enzymology/*metabolism/pathology| Endometrium/enzymology/*metabolism/pathology| Estradiol/metabolism| Estrogens/*metabolism| Female| Fertility/physiology| Humans |
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